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Bacteria



BCRC Number: 80529
Organism: Rhodovulum euryhalinum
Synonymy: Rhodobacter euryhalinus
Author: (Kompantseva 1989) Hiraishi and Ueda
History: << DSMZ << J. Imhoff << Gorlenko << Kompantseva, strain KA-65
Other Collection No.: DSM 4868
Source: shallow saline waters
Others: 1.冷凍保存法:抗凍劑為 5% DMSO (最終濃度)。 2.冷凍小管只能解凍一次並且完全活化,不可重複冷凍解凍。 3.菌株復活:冷凍小管立即置於37-40℃水浴中,不時搖動,使冷凍菌液完全融解。回溶的菌液必須全部吸取至 10 ml 的新鮮培養基中進行活化培養。 4.特殊培養條件:厭氧和光照。 5.增殖培養的接種量需求:接種菌液的體積必須佔新鮮培養基的 20% 至 50%。
Growth Conditions: 30°C 7 day(s)
Biosafety Level: 1
Oxygen Requirement: Anaerobic
Type Strain: YES
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References



B4508
Hiraishi, A. and Y. Ueda. 1994.. Intrageneric structure of the genus Rhodobacter: transfer of Rhodobacter sulfidophilus and related marine species to the genus Rhodovulum gen. nov. Int. J. Syst. Bacteriol. 44 : 15-23.
 

Medium



904
RHODOSPIRILLACEAE MEDIUM (modified)
(NH4)-acetate0.50 g
CaCl2 · 2 H2O0.05 g
Distilled water1000 mL
Fe(III) citrate solution (0.1% in H2O)5.00 mL
KH2PO40.50 g
L-Cysteiniumchloride0.30 g
MgSO4 · 7 H2O3.0 g
Na2-succinate1.00 g
NaCl30.0 g
NH4Cl0.40 g
Resazurin (0.1%)0.50 mL
Trace element solution SL-6 (see below)1.00 mL
Vitamin B12 solution (10 mg in 100 mL H2O)0.40 mL
Yeast extract0.30 g
Refer to BCRC medium 903, increase the NaCl to 30 g (per liter) and increase the MgSO4· 7H2O to 3 g (per liter).
Trace element solution SL-6
CoCl2· 6 H2O0.20 g
CuCl2 ·2 H2O0.01 g
Distilled water1000 mL
H3BO30.30 g
MnCl2·4 H2O0.03 g
Na2MoO4·2 H2O0.03 g
NiCl2· 6 H2O0.02 g
ZnSO4·7 H2O0.10 g

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