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Bacteria



BCRC Number: 80528
Organism: Rhodovulum visakhapatnamense
Author: Srinivas et al.
History: << DSMZ << C. Sasikala, J. N. T. Univ., Inst. Sci. Technol., Hyderabad, India; JA 181 << P. A. Kumar
Other Collection No.: DSM 17937; ATCC BAA-1274; JCM 13531
Source: marine surface and tidal waters exposed to light
Isolated Info:
    Isolated location: India, eastern coast, Bay of Bengal, Ramakrishna beach at Visakhapatnam (17° 42' N 83° 18' E)
Others: 1.冷凍保存法:抗凍劑為 5% DMSO (最終濃度)。 2.冷凍小管只能解凍一次並且完全活化,不可重複冷凍解凍。 3.菌株復活:冷凍小管立即置於37-40℃水浴中,不時搖動,使冷凍菌液完全融解。回溶的菌液必須全部吸取至 10 ml 的新鮮培養基中進行活化培養。 4.特殊培養條件:厭氧和光照。 5.增殖培養的接種量需求:接種菌液的體積必須佔新鮮培養基的 20% 至 50%。
Growth Conditions: 25°C 7 day(s)
Biosafety Level: 1
Oxygen Requirement: Anaerobic
Type Strain: YES
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References



B4507
Srinivas, T. N. R., P. A. Kumar, C. Sasikala, C. Ramana, and J. F. Imhoff. 2007.. Rhodovulum visakhapatnamense sp. nov. Int. J. Syst. Evol. Microbiol. 57 : 1762-1764.
 

Medium



905
RHODOVULUM VISAKHUM MEDIUM
NaCl20.0 g
NH4Cl0.60 g
CaCl2· 2 H2O0.15 g
Yeast extract0.40 g
Sodium pyruvate 3.0 g
Distilled water1000 mL
KH2PO40.50 g
MgCl2 ·7 H2O1.00 g
Adjust to pH 6.8. Autoclave the medium and Trace element solution SL8 (see below) separately. Aseptically add 1.0 mL Trace element solution SL8 and 1.0 mL Vitamin B12 (2 mg/L concentration, filter sterilised) to the medium after autoclaving.
Trace element solution SL8
Na2· EDTA5.2 g
FeCl2·4 H2O1.5 g
ZnCl270.0 mg
MnCl2 · 4 H2O0.1 g
H3BO362.0 mg
CoCl2 · 6 H2O190.0 mg
CuCl2 · 2 H2O17.0 mg
NiCl2 · 6 H2O24.0 mg
Na2MoO4 · 2 H2O36.0 mg
Distilled water1000 mL
Adjust to pH 6.5.

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