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Bacteria



BCRC Number: 80527
Organism: Rhodopseudomonas rhenobacensis
Author: Hougardy et al.
History: << DSMZ << J.-H. Klemme, R1
Other Collection No.: DSM 12706; CIP 106682
Source: sediment of eutrophic pond
Isolated Info:
    Isolated location: Germany, Rheinbach
Others: 1.冷凍保存法:抗凍劑為 5% DMSO (最終濃度)。
2.冷凍小管只能解凍一次並且完全活化,不可重複冷凍解凍。
3.菌株復活:冷凍小管立即置於37-40℃水浴中,不時搖動,使冷凍菌液完全融解。回溶的菌液必須全部吸取至 10 ml 的新鮮培養基中進行活化培養。
5.使用 Medium 996 需在厭氧照光(tungsten lamp)環境下培養。
6.增殖培養的接種量需求:接種菌液的體積必須佔新鮮培養基的 20% 至 50%。
7.使用 Medium 66 在好氧黑暗環境下培養。
Growth Conditions: 28°C 7 day(s)
Biosafety Level: 1
Oxygen Requirement: Anaerobic
Type Strain: YES
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References



B4513
Hougardy, A., B. J. Tindall, and J. H. Klemme. 2000. Rhodopseudomonas rhenobacensis sp. nov., a new nitrate-reducing purple non-sulfur bacterium. Int.J.Syst.Evol.Microbiol. 50 : 985-992.
 

Medium



66
TRYPTIC SOY BROTH OR TRYPTIC SOY AGAR (DIFCO 0369)
Tryptone15.0 g
Soytone5.0 g
NaCl5.0 g
Agar15.0 g
Distilled water1.0 L
Adjust pH to 7.3.
996
RHODOSPIRILLACEAE MEDIUM (reduce yeast extract and pH)
(NH4)-acetate0.50 g
CaCl2 · 2 H2O0.05 g
Distilled water1000 mL
Fe(III) citrate solution (0.1% in H2O)5.00 mL
KH2PO40.50 g
L-Cysteiniumchloride0.30 g
MgSO4 · 7 H2O0.40 g
Na2-succinate1.00 g
NaCl0.40 g
NH4Cl0.40 g
Resazurin (0.1%)0.50 mL
Trace element solution SL-6 (see below)1.00 mL
Vitamin B12 solution (10 mg in 100 mL H2O)0.40 mL
Yeast extract0.20 g
Reduce Medium 903 the yeast extract concentration to 0.2 g/L. Boil the medium for a few minute. Bubble the medium with nitrogen gas and fill 10 mL in 15 mL tubes with a rubber septum under a stream of nitrogen gas. Autoclave at 121°C for 15 min. Sterile syringes are used to inoculate and remove samples. Incubate in the light using a tungsten lamp. Adjust pH to 5.7.
Trace element solution SL-6
CoCl2· 6 H2O0.20 g
CuCl2 ·2 H2O0.01 g
Distilled water1000 mL
H3BO30.30 g
MnCl2·4 H2O0.03 g
Na2MoO4·2 H2O0.03 g
NiCl2· 6 H2O0.02 g
ZnSO4·7 H2O0.10 g

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