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Bacteria



BCRC Number: 80526
Organism: Rhodopseudomonas julia
Author: Kompantseva
History: << DSMZ << ATCC << J. F. Imhoff, KR-11-67 << E. I. Kompantseva
Other Collection No.: ATCC 51105; DSM 11549
Source: sulfur spring
Others: 1.冷凍保存法:抗凍劑為 5% DMSO (最終濃度)。 2.冷凍小管只能解凍一次並且完全活化,不可重複冷凍解凍。 3.菌株復活:冷凍小管立即置於37-40℃水浴中,不時搖動,使冷凍菌液完全融解。回溶的菌液必須全部吸取至 10 ml 的新鮮培養基中進行活化培養。 4.特殊培養條件:厭氧和光照。 5.增殖培養的接種量需求:接種菌液的體積必須佔新鮮培養基的 20% 至 50%。
Growth Conditions: 25°C 7 day(s)
Biosafety Level: 1
Oxygen Requirement: Anaerobic
Type Strain: YES
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References



B4505
Kompantseva, E. I. 1989.. A new species of budding purple bacterium: Rhodopseudomonas julia sp. nov. Mikrobiologiya 58: 319-325.
B4506
Anonymus 1993.. Validation of the publication of new names and new combinations previously effectively published outside the IJSB. List No. 44 Int. J. Syst. Bacteriol. 43 : 188-189.
 

Medium



903
RHODOSPIRILLACEAE MEDIUM (modified)
Na2-succinate1.00 g
(NH4)-acetate0.50 g
Fe(III) citrate solution (0.1% in H2O)5.00 mL
KH2PO40.50 g
MgSO4 · 7 H2O0.40 g
NaCl0.40 g
NH4Cl0.40 g
CaCl2 · 2 H2O0.05 g
Vitamin B12 solution (10 mg in 100 mL H2O)0.40 mL
Trace element solution SL-6 (see below)1.00 mL
Resazurin (0.1%)0.50 mL
Distilled water1000 mL
Yeast extract0.30 g
L-Cysteiniumchloride0.30 g
Adjust pH to 6.8. Boil the medium for a few minute. Bubble the medium with nitrogen gas and fill 10 ml in 15 ml tubes with a rubber septum under a stream of nitrogen gas. Autoclave at 121°C for 15 min. Sterile syringes are used to inoculate and remove samples. Incubate in the light using a tungsten lamp.
Trace element solution SL-6
ZnSO4·7 H2O0.10 g
MnCl2·4 H2O0.03 g
H3BO30.30 g
CoCl2· 6 H2O0.20 g
CuCl2 ·2 H2O0.01 g
NiCl2· 6 H2O0.02 g
Na2MoO4·2 H2O0.03 g
Distilled water1000 mL

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