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Bacteria



BCRC Number: 80431
Organism: Clostridium butyricum
Author: Prazmowski
History: << NBRC << IFO << ATCC << NCTC << J. Howie, strain Rowett
Other Collection No.: ATCC 19398; DSM 10702; IAM 14194; IFO 13949; JCM 1391; NBRC 13949; NCIB 7423; NCTC 7423; VPI 3266
Source: intestine of pig
Others: 原菌種中心NBRC建議培養基為53。經測試72與53皆可生長,為方便顧客培養,故並列2個培養基。
Growth Conditions: 37°C
Biosafety Level: 1
Oxygen Requirement: Anaerobic
Type Strain: YES
    Reference: B1
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References



B1
Skerman, V. B. D., V. McGowan, and P. H. A. Sneath. 1980. Approved lists of bacterial names. Int. J. Syst. Bacteriol. 30 : 225–420.
 

Medium



53
REINFORCED CLOSTRIDIAL MEDIUM (OXOID CM149)
'Lab-lemco' powder10.0 g
Peptone10.0 g
Cysteine·HCl0.5 g
NaCl5.0 g
CH3COONa3.0 g
Soluble starch1.0 g
Glucose5.0 g
Yeast extract3.0 g
Agar0.5 g
Distilled water1.0 L
Adjust pH to 6.8. Bring to the boil to dissolve completely. Autoclave at 121°C/15 min.
72
TRYPTIC SOY AGAR with 5% Defibrinated Sheep Blood
Agar15.0 g
Defibrinated Sheep Blood50.0 mL
Distilled water1.0 L
NaCl5.0 g
Soytone5.0 g
Tryptone15.0 g

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