Bacteria



BCRC Number: 80395
Organism: Xanthomonas campestris pv. campestris
Author: (Pammel) Dowson
History: << NCHU, N. T. Hu, XC1763
Genotype: ΔxpsG,xpsE-ecfp
Growth Conditions: 28°C 2 day(s)
Biosafety Level: 1
Regulation Authority: Bureau of Animal and Plant Health Inspection and Quarantine
Shipping/Field/Price:
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References



 

Medium



853
PYX-MEDIUM
Trypticase peptone5 g
Peptone from meat (pepsin-digested)5 g
Yeast extract10 g
Glucose5 g
Resazurin1 mg
Salt solution40 ml
Cysteine-HCl x H2O0.5 g
Distilled water1000 ml
Dissolve ingredients (except glucose and cysteine), boil medium for 1 min., then cool to room temperature under N2 gas atmosphere. Add cysteine and adjust pH to 7.0. Thereafter, dispense under N2 gas atmosphere in culture vessels and autoclave. After autoclaving add glucose or any other substrate from a sterile, anoxic stock solution prepared under N2.
Salt solution
CaCl2x 2 H2O0.25 g
MgSO4 x 7 H2O0.50 g
K2HPO41.00 g
KH2PO41.00 g
NaHCO310.00 g
NaCl2.00 g
Distilled water1000.00 ml

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