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Bacteria



BCRC Number: 80380
Organism: Magnetospirillum magnetotacticum
Author: (Maratea and Blakemore) Schleifer et al.
History: << NBRC << IFO << DSMZ << ATCC 31632 << R. P. Blakemore, MS-1
Other Collection No.: ATCC 31632; DSM 3856; NBRC 15272; IAM 14723; LMG 10894; JCM 21281
Source: freshwater sediment
Others: ◆(2015-01-06)經測試後,於611號培養基生長良好,建議可用此培養。
Growth Conditions: 30°C
Biosafety Level: 1
Oxygen Requirement: Microaerophilic
Type Strain: YES
    Reference: B3626
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References



B3626
Schleifer, K. H., D. Schuler, S. Spring, M. Weizenegger, R. Amann, W. Ludwig, and M. Kohler. 1991. The genus Magnetospirillum gen. nov. description of Magnetospirillum gryphiswaldense sp. nov. and transfer of Aquaspirillum magnetotacticum to Magnetospirillum magnetotacticum comb. nov. Syst. Appl. Microbiol. 14 : 379–385.
 

Medium



611
Magntospirillum medium
KH2PO40.68g
Na-thioglycolate0.05g
L(+)-Tartaric acid0.37g
Succinic acid0.37g
Trace elements solution5ml
Resazurin0.5mg
Distilled water1000ml
NaNO30.12g
Na-acetate0.05g
Vitamin solution10ml
Fe(Ⅲ) quinate solution2ml
Dissolve components in the order given. Adjust pH to 6.75 with NaOH and boil medium for 1 min. Purge medium with N2 gas for 10 min. Under the same atmosphere, anaerobically fill tubes to 1/3 of their volume and seal. Autoclave at 121℃ for 15 min. Before inoculation, ad
Trace elements solution
Nitrilotriacetic acid1.5g
MgSO4·7 H2O3g
MnSO4·2 H2O0.5g
NaCl1g
CaCl2·2 H2O0.1g
Zn SO4·7 H2O0.18g
Cu SO4·5 H2O0.01g
Kal(SO4)2·12 H2O0.02g
H3BO30.01g
Na2MoO4·2 H2O0.01g
Distilled water1000ml
FeSO4·7 H2O0.1g
CoSO4·7 H2O0.18g
NiCl2·6 H2O0.025g
Na2SeO3·5 H2O0.3mg
Na2WO4·2 H2O0.4 mg
Trace elements solution:First dissolve nitrilotriacetic acid adjust pH to 6.5 with KOH, then add minerals. Final pH 7.0 (with KOH).

Vitamin solution
Biotin2mg
Folic acid2mg
Pyridoxine-HCl10mg
Thiamine-HCl·2 H2O5mg
Riboflavin5mg
Vitamin B120.1mg
p-Aminobenzoic acid5mg
Lipoic acid5mg
Distilled water1000ml
Nicotinic acid5mg
D-Ca-pantothenate5mg

Ferric quinate solution, 0.01 M
FeCl3·6H2O0.45g
Quinic acid0.19g
Distilled water100ml
Ferric quinate solution:Dissolve and autoclave at 121℃ for 15 min.

858
Magnetospirillum medium
KH2PO40.28 g
NaNO30.12 g
Sodium thioglycolate0.05 g
Sodium tartrate0.37 g
Sodium succinate0.37 g
Sodium acetate0.05 g
Vitamin mixture5 ml
Ferric quinate solution2 ml
HMSS solution1 ml
Distilled water1 L
Adjust pH to 6.75 with 1 N NaOH. Good growth can be obtained in semi-solid medium (1 g of agar in 1 liter).
Vitamin mixture
Biotin0.2 mg
Ca-pantothenate40 mg
Folic acid0.2 mg
/p/-Aminobenzoic acid20 mg
Niacin40 mg
myo-Inositol200 mg
Pyridoxine-HCl40 mg
Thiamin-HCl20 mg
Cyanocobalamin2 μg
Distilled water1 L
Choline chloride100 mg
Riboflavin40 mg

Ferric quinate solution
FeCl3·6H2O0.45 g
Quinic acid0.19 g
Distilled water1 L

HMSS solution
FeSO4·7H2O99 mg
Na2MoO4·2H2O12.7 mg
CaCl2·2H2O3.3 g
Metal 44 solution50 ml
Distilled water1 L
Nitrilotriacetic acid10 g
MgSO4·7H2O29.7 g
Neutralize the nitrilotriacetic acid with KOH. Dissolve the remaining ingredients and adjust the pH to 7.2 with KOH.

Metal 44 solution
EDTA2.5 g
Distilled water1 L
Na2B4O7·10H2O0.177 g
FeSO4·7H2O5 g
MnSO4·nH2O1.54 g
CoCl2·6H2O0.203 g
CuSO4·5H2O0.392 g
ZnSO4·7H2O10.95 g

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