Bacteria



BCRC Number: 80375
Organism: Aggregatibacter actinomycetemcomitans
Synonymy: Haemophilus actinomycetemcomitans
Author: Norskov-Lauritsen and Kilian
History: << NCTC (Haemophilus actinomycetemcomitans) << P. Holm. Abscess; Denmark. Serovar c.
Other Collection No.: NCTC 9710; DSM 8324; ATCC 33384; CCUG 13227; CIP 52.106; BCRC14407
Source: abscess
Isolated Info:
    Isolated location: Denmark
Characterization:
Taxonomy --B4433,B4435,B4437
Growth Conditions: 37°C 3 day(s)
Biosafety Level: 2
Oxygen Requirement: 5% CO2
Type Strain: YES
    Reference: B4437
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References



B4433
Potts,T.V., J. J. Zambon, and R. J. Genco. 1985. Reassignment of Actinobacillus actinomycetemcomitans to the genus Haemophilus as Haemophilus actinomycetemcomitans comb.nov. Int.J.Syst.Bacteriol. 35: 337-341.
B4435
de Ley, J., W. Mannheim, R. Mutters, K. Piechulla, R. Tytgat, P. Segers, M. Bisgaard, W. Frederiksen, K. H. Hinz, and M. Vanhoucke. 1990. Inter- and intrafamilial similarities of rRNA cistrons of the Pasteurellaceae. Int.J.Syst.Bacteriol. 40: 126-137.
B4437
Norskov-Lauritsen, N., and M. Kilian. 2006. Reclassification of Actinobacillus actinomycetemcomitans, Haemophilus aphrophilus, Haemophilus paraphrophilus and Haemophilus segnis as Aggregatibacter actinomycetemcomitans gen. nov., comb. nov., Aggregatibacter aphrophilus comb. nov. and Aggregatibacter segnis comb. nov., and emended description of Aggregatibacter aphrophilus to include V factor-dependent and V factor-independent isolates. Int.J.Syst.Evol.Microbiol. 56: 2135-2146.
 

Medium



4
BRAIN HEART INFUSION AGAR (DIFCO 0418)
Calf brain, infusion from 200.0 g7.7 g
Beef heart, infusion from 250.0 g9.8g
Proteose peptone10.0 g
Dextrose2.0 g
NaCl5.0 g
Distilled water1.0 L
Na2HPO42.5 g
Agar15.0 g
Adjust pH to 7.4.
44
BLOOD AGAR
Agar15.0g
Beef heart, infusion from500.0g
Distilled water1.0L
NaCl5.0g
Tryptose10.0g
Prepare Blood Agar Base according to directions on the bottle. Sterilize and cool to about 45°C. Aseptically add 5% sterile defibrinated rabbit blood. Mix and quickly dispense into sterile test tubes. Slant.

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