Bacteria



BCRC Number: 80285
Organism: Bacillus velezensis
Author: Ruiz-Garcia et al.
History: << NCCB (Bacillus methylotrophicus) << Dept Agricultural Chemistry Chungbuk National University, Korea, M. Madhaiyan, CBMB205
Other Collection No.: NCCB 100236; KACC 13105
Source: rhizosphere soil cultivated rice
Others: ◆(2016-09-01)Type strain of Bacillus methylotrophicus/--B4351
◆(2016-09-01)Rename as Reference--B2381
Growth Conditions: 30°C
Biosafety Level: 1
Type Strain: NO
    Reference: B4351,B2381
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References



B2381
Dunlap, C. A. , S. J. Kim , S. W. Kwon , and A. P. Rooney 2016. Bacillus velezensis is not a later heterotypic synonym of Bacillus amyloliquefaciens; Bacillus methylotrophicus, Bacillus amyloliquefaciens subsp plantarum and 'Bacillus oryzicola' are later heterotypic synonyms of Bacillus velezensis based on phylogenomics. Int J Syst Evol Microbiol. 66 : 1212-1217.
B4351
Madhaiyan, M., S. Poonguzhali, S. W. Kwon, and T. M. Sa. 2010. Bacillus methylotrophicus sp. nov., a methanol-utilizing, plant-growth-promoting bacterium isolated from rice rhizosphere soil. Int. J. Syst. Evol. Microbiol. 60 : 2490–2495.
 

Medium



675
Trypticase Soy Broth (BBL 211768) or Trypticase Soy Agar
Pancreatic digest of casein 17 g
Papaic digest of soybean3 g
Sodium chloride 5 g
Dipotassium phosphate 2.5 g
Dextrose2.5 g
Distilled water 1 L
Warm gently until solution is complete. Sterilize by autoclaving at 121°C for 15 minutes. If needed, add 15 g agar per liter medium.
842
AMS (Ammonium Mineral Salts) Medium
K2HPO40.7 g
MgSO4·7H2O1.0 g
CaCl2·2H2O0.2 g
FeSO4·7H2O4 mg
ZnSO4·7H2O100 μg
MnCl230 μg
CoCl2·6H2O200 μg
CuCl2·2H2O10 μg
Na2MoO4·2H2O60 μg
Agar (if needed)15 g
Distilled water1.0 L
KH2PO40.54 g
NH4Cl0.5 g
H3BO3300 μg
NiCl2·6H2O20 μg
Adjust pH to 6.8. After sterilization, add filter-sterilized methanol in a 1:50 ratio to medium.

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