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Bacteria



BCRC Number: 80218
Organism: Thermoanaerobacter wiegelii
Author: Cook et al.
History: << DSM << H. Morgan << B. K. Patel, Rt8.B1 (TG30)
Other Collection No.: DSM 10319
Source: freshwater pool, geothermal heat exchanger
Isolated Info:
    Isolated location: New Zealand, Rotorua
Characterization:
Taxonomy --B4282
Growth Conditions: 65°C 3 day(s)
Biosafety Level: 1
Oxygen Requirement: Anaerobic
Type Strain: YES
    Reference: B4282
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References



B4282
Cook, G. M., F. A. Rainey, B. K. C. Patel, and H. W. Morgan. 1996. Characterization of a new obligately anaerobic thermophile, Thermoanaerobacter wiegelii sp. nov. Int. J. Syst. Bacteriol. 46 : 123–127.
 

Medium



832
CALDICELLULOSIRUPTOR MEDIUM WITH 0.5% GLUCOSE
NH4Cl0.90 g
NaCl0.90 g
MgCl2 x 6 H2O 0.40 g
KH2PO40.75 g
Trypticase peptone2.00 g
Yeast extract1.00 g
Trace element solution SL-10 (see below)1.00 ml
FeCl3 x 6 H2O 2.50 mg
Cellobiose or Cellulose1.00 g
Cysteine-HCl x H2O0.75 g
Distilled water1000.00 ml
K2HPO41.50 g
Resazurin0.50 mg
Adjust pH to 7.2. Prepare medium anaerobically under 100% N2.
Trace element solution SL-10
HCl (25%; 7.7M)10.0ml
FeCl2 x 4H2O1.5g
ZnCl270.0mg
MnCl2 x 4H2O100.0mg
CoCl2 x 6H2O190.mg
CuCl2 x 2H2O2.0mg
NiCl2 x 6H2O24.0mg
Na2MoO4 x 2H2O36.0mg
Distilled water990.ml
H3BO36.0mg
First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.0mL

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