Bacteria



BCRC Number: 80146
Organism: Komagataeibacter xylinus
Author: (Brown 1886) Yamada et al. 2013
History: << LMG << NCIB << NCTC << O. Verona (Gluconacetobacter europaeus)
Other Collection No.: LMG 1518; ATCC 11142; CECT 473; DSM 2325; LMD 75.1; LMG 1538; NCIB 5346; NCTC 5346
Others: 1.(2017-09-21)16S rDNA sequence similarities were most closely related to Komagataeibacter europaeus/(100%), K. xylinus/(100%) and K. swingsii/(99.9%).
2.(2018-11-27)Rename as LMG 1518.
3.(2018-11-28)Rename as Reference B414
Growth Conditions: 28°C 8 day(s)
Biosafety Level: 1
Oxygen Requirement: Aerobic
Type Strain: NO
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References



B414
CristinaAndrés-Barrao, CinziaBenagli, MalouChappuis, RubenOrtega Pérez, MauroTonolla, FrançoisBarja 2013. Rapid identification of acetic acid bacteria using MALDI-TOF mass spectrometry fingerprinting Syst. Appl. Microbiol. 36: 75-81.
 

Medium



530
RAE MEDIUM
Glucose40.00g
Peptone10.00g
Yeast extract10.00g
Citric acid·H2O1.50g
Na2HPO4·2H2O3.38g
Glacial acetic acid10.00ml
Absolute ethanol10.00ml
Distilled water980.00ml
Final pH ca. 3.8 (pH is not adjusted) The ethanol and acetic acid are sterilized separately by filtration and added aseptically to the sterile medium. A double layer system has to be used. A layer of growth medium containing 1% agar is poured into a plate and allowed to solidify. A thin layer of the growth medium containing 2% agar is then poured on top.

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