Bacteria



BCRC Number: 80045
Organism: Bacillus subtilis subsp. spizizenii
Author: Nakamura et al.
History: << DSMZ << ATCC << N. R. Smith, 231 << K. F. Kellermann.
Other Collection No.: DSM 347; ATCC 6633; NRRL B-765; CCM 1999; IAM 1069; NCIB 8054; NCTC 10400; BCRC 10447
Characterization:
Assay antibiotics -- B4058,B4059,B4055
Production bacilysin and fengymycin -- B4063
restriction endonuclease Bsu 6633 -- B3909
Test Test for antimicrobial compounds in paper, cardboard, etc.
strain for growth media, sterility testing -- B4057
Taxonomy --B4053,B4054,PMID15128533
Others: 1.Resistant to nisin -- B4056.
2.Peptide antibiotics -- B4062.
3.Quality control strain according to DIN 58959-7 and European Pharmacopoeia -- B4059.
4.菌落有2種型態,一為米白色,一為米黃色粘稠,經原菌種中心回覆此現象為正常。
Growth Conditions: 30°C 2 day(s)
Biosafety Level: 1
Oxygen Requirement: Aerobic
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References



B3909
Roberts, R. J. 1985. Restriction and modification enzymes and their recognition sequences Nucl. Acids Res. 13: 165–200.
B4053
Lovett, P. S.,and P. E. Young. 1969. Identification of Bacillus subtilis NRRL B-3275 as a strain of Bacillus pumilus. J. Bacteriol. 100: 658-661.
B4054
Dubnau, D., I. Smith, P. Morrel, and J. Marmur. 1965. Gene conservation in Bacillus species. I. Conserved genetic and nucleic base sequence homologies. Proc. Natl. Acad. Sci. U. S. A. 54: 491-498.
B4055
Tittiger, F., B. Kingscote, B. Meldrum, and M. Prior. 1975. Survey of antibiotic residues in Canadian slaughter animals. Can. J. Comp. Med. 39: 178-182.
B4057
2000. European Pharmacopeia. 2.6.1. Sterility Testing. 2.6.12. and 2.6.13 Microbiological testing of non-sterile products. Deutscher Apotheker Verlag, Stuttgart. 51-65:
B4058
1973. Handbook of Microbiology Vol.III. Microbial products. CRC Press.
B4059
2000. European Pharmacopoeia. 2.7.2 Antibiotica assays. Deutscher Apotheker-Verlag, Stuttgart. 85-90:
B4063
Loeffler, W., J. S. M. Tschen, N. Vanittanakom, M. Kugler, E. Knorpp, T. F. Hsieh, and T. G. Wu. 1986. antifungal effects of bacilysin and fengymycin from Bacillus subtilis F-29-3. A comparison with activities of other Bacillus antibiotics. J. Phytopathol. 115: 204-213.
PMID15128533
Burke SA, Wright JD, Robinson MK, Bronk BV, Warren RL. 2004 May. Detection of molecular diversity in Bacillus atrophaeus by amplified fragment length polymorphism analysis. Applied and environmental microbiology 70(5): 2786-90.
 

Medium



3
NUTRIENT AGAR (DIFCO 0001)
Beef extract3.0 g
Peptone5.0 g
Agar15.0 g
Distilled water1.0 L
Adjust pH to 7.0.

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