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Bacteria



BCRC Number: 80008
Organism: Sulfobacillus thermosulfidooxidans
Author: Golovacheva and Karavaiko
History: << DSMZ
Other Collection No.: DSM 9293; VKM B-1269
Source: spontaneously heated ore deposit
Growth Conditions: 50°C 5 day(s)
Biosafety Level: 1
Oxygen Requirement: Aerobic
Type Strain: YES
    Reference: B4007
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References



B4007
Golovacheva, R. S., and G. I. Karavaiko. 1978. A new genus of thermophilic spore-forming bacteria, Sulfobacillus. Microbiology. 47 : 658–664.
B4008
International Committee on Systematic Bacteriology. 1991. Validation list No. 36 Int. J. Syst. Bacteriol. 41 : 178–179.
 

Medium



803
SULFOBACILLUS MEDIUM
Solution A700ml
Solution B300ml
Solution C20ml
combine solution A,B and C together after autoclaving. Adjust pH to 1.9~2.4.
Solution A
(NH4)2SO43.0g
KCl0.1g
K2HPO40.5g
MgSO4·7H2O0.5g
Ca(NO3)20.01g
Distilled water700ml
Adjust pH to 2.0~2.2 with sulfuric acid. Sterilized by 121℃, 15min.

Solution B
10N H2SO41ml
FeSO4·7H2O44.2g
Distilled water 300ml
Sterilized by 121℃, 15min.

Solution C
Yeast extract0.2g
Distilled water20ml
Sterilized by 121℃, 15min.

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