Cell Line



BCRC Number: 60566 Add To Bookmark
Name: OP9
Type: bone marrow, stroma
Species: Mus musculus/(mouse)
Strain Ethnicity: (C57BL/6 x C3H)F2 -op/op
Age Stage: newborn
Gender: unknown
Tissue: stroma

Morphology: fibroblast
Split Ratio: Maintain cultures at a cell concentration between 4 X 10(3) and 1 X 10(4) cells/cm2.
Mycoplasma Test: negative
Doubling time: 26 hrs

Incubation Temp(°C): 37°C
Incubation Condition: 5%CO2
Culture Medium: Alpha minimum essential medium without ribonucleosides and deoxyribonucleosides with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 80%; fetal bovine serum, 20%
Growth Property: adherent
SubCulture Procedure: by trypsin-EDTA
Freezed Medium: 93% culture medium+7% DMSO
Seeding Density: A subcultivation ratio of 1:4 to 1:5 is recommended, Maintain cultures at a cell concentration between 4 X 10(3) and 1 X 10(4) cells/cm2.
Medium Renewal: every 2 to 3 days


Image / Description:   100倍視野下低細胞密度
  100倍視野下高細胞密度
 

Biosafety Level: 1
Reference: Proc. Natl. Acad. Sci. USA 99: 12819-12824, 2002, Science 265: 1098-1101, 1994
Shipping/Field/Price:
Frozen Vial for all NTD$ 7000 Order this item

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