Cell Line



BCRC Number: 60556
Name: A20
Type: B cell lyphoma, immunoglobulin (surface, sIg+)
Species: Mus musculus (mouse)
Strain Ethnicity: BALB/cAnN
Gender: unknown
Tissue: B lymphocyte; reticulum cell sarcoma

Morphology: lymphoblast
Split Ratio: Start cultures at 2 x 10 exp5 cells/ml and maintain between 1 x 10 exp5 and 1 x 10 exp6 cells/ml.
Mycoplasma Test: negative

Incubation Temp(°C): 37°C
Incubation Condition: 5%CO2
Culture Medium: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate and supplemented with 0.05 mM 2-mercaptoethanol, 90%; fetal bovine serum, 10%
Growth Property: suspension(細胞部分會貼附,可用medium沖下來,沖不下來的細胞就不要了)
SubCulture Procedure: Cultures can be maintained by addition or replacement of fresh medium.
Freezed Medium: 93%culture medium+7%DMSO
Seeding Density: Start cultures at 2 x 10 exp5 cells/ml and maintain between 1 x 10 exp5 and 1 x 10 exp6 cells/ml.
Medium Renewal: every 2 to 3 days

Description: The A20 cell line is a BALB/c B cell lymphoma line derived from a spontaneous reticulum cell neoplasm found in an old BALB/cAnN mouse.

Image / Description:   200倍視野下
 

Biosafety Level: 1
Reference: J. Immunol. 122: 549-554, 1979, J. Exp. Med. 155: 445-459, 1982, Proc. Natl. Acad. Sci. USA 93: 11047-11052, 1996
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