Cell Line



BCRC Number: 60554 Add To Bookmark
Name: C-33A
Type: cervical carcinoma
Species: Homo sapiens (human)
Age Stage: 66 years
Gender: Female
Tissue: cervix

Morphology: epithelial
Split Ratio: 1:3 to 1:8
Mycoplasma Test: negative

Incubation Temp(°C): 37°C
Incubation Condition: 5% CO2
Culture Medium: Minimum essential medium (Eagle) with 2 mM L-glutamine and Earle's BSS adjusted to contain 1.5 g/L sodium bicarbonate, 0.1 mM non-essential amino acids, and 1.0 mM sodium pyruvate, 90%; fetal bovine serum, 10%
Growth Property: adherent
SubCulture Procedure: by trypsin-EDTA
Freezed Medium: 93%culture medium+7%DMSO
Medium Renewal: every 2 to 3 days

Description: The C-33 A cell line is one of a series of lines derived by N. Auersperg from cervical cancer biopsies.The cells are negative for human papillomavirus DNA and RNA.
Others: STR-PCR profile — D7S820:10 CSF1PO:12 TH01:7, 8 D13S317:13 D16S539:13, 14 vWA:18, 20 TPOX:9 Amelogenin:X D5S818:11, 12

Image / Description:   200倍視野下
  100倍視野下
  200倍視野下
 

Biosafety Level: 1
Reference: J. Natl. Cancer Inst. 32: 135-148, 1964, Am. J. Pathol. 119: 361-366, 1985, Proc. Natl. Acad. Sci. USA 88: 5523-5527, 1991
Shipping/Field/Price:
Frozen Vial for all NTD$ 7000 Order this item

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