Cell Line



BCRC Number: 60553
Name: NAMALWA
Type: Burkitt's lymphoma
Species: Homo sapiens/human
Age Stage: years
Gender: Female

Morphology: lymphoblast
Split Ratio: Cultures should be started at 5 X 10(5) viable cells/ml and subcultured at 2 X 10(6) cells/ml.
Mycoplasma Test: negative

Incubation Temp(°C): 37°C
Incubation Condition: 5% CO2
Culture Medium: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate, 92.5%; fetal bovine serum, 7.5%
Growth Property: suspension
SubCulture Procedure: Cultures can be maintained by addition or replacement of fresh medium.
Freezed Medium: 93% culture medium+7% DMSO
Seeding Density: Cultures should be started at 5 X 10(5) viable cells/ml and subcultured at 2 X 10(6) cells/ml.
Medium Renewal: every 2 to 3 days

Description: The cells contain the Epstein-Barr virus (EBV) genome.
Others: STR-PCR profile — D7S820:11 CSF1PO:10,11 TH01:7,9.3 D13S317:11,12 D16S539:9 vWA:14 TPOX:6,11 Amelogenin:X D5S818:12,13
Product Character: IgM; lambda light chain

Image / Description:   200倍視野下
 

Biosafety Level: 2
Reference: Antimicrob. Agents Chemother. 5: 420-427, 1979, J. Immunol. 129: 1336-1342, 1982, Int. J. Cancer 10: 44-57, 1972, Int. J. Cancer 12: 396-408, 1973.
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