Cell Line

BCRC Number: 60552
Name: HEC-1-A
Type: Uterus,endometrium,adenocarcinoma
Species: Homo sapiens/human
Age Stage: 71 years
Gender: Female
Tissue: Uterus

Morphology: epithelial
Split Ratio: 1:4 to 1:8
Isoenzyme Analysis: AK-1, 1; ES-D, 1; G6PD, B; GLO-I, 2; Me-2, 1; PGM1, 1; PGM3, 1-2
Mycoplasma Test: negative
Tumorigenic: in nude mice, in the cheek pouch of cortisone treated hamsters
Receptor expressed: platelet activating factor (PAF)

Incubation Temp(°C): 37°C
Incubation Condition: 5%CO2
Culture Medium: McCoy's 5a medium (modified) with 1.5 mM L-glutamine adjusted to contain 2.2 g/L sodium bicarbonate, 90%; fetal bovine serum, 10%
Growth Property: adherent
SubCulture Procedure: by trypsin-EDTA
Freezed Medium: 93% cluture medium+7%DMSO
Medium Renewal: Every 2 to 3 days

Description: PAF induces increased expression of c-fos.
Others: STR-PCR profile — D7S820:9,11 CSF1PO:10,12 TH01:6,7 D13S317:11 D16S539:12 vWA:18,19 TPOX:8,11 Amelogenin:X D5S818:11,15

Image / Description:   200倍視野下低細胞密度

Biosafety Level: 1
Reference: Acta Obstet. Gynaecol. Jpn. 19: 47-58, 1972, J. Natl. Cancer Inst. 58: 209-214, 1977,J. Natl. Cancer Inst. 59: 221-226, 1977
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