Cell Line



BCRC Number: 60551 Add To Bookmark
Name: NIH:OVCAR-3
Type: ovary, adenocarcinoma,epithelial
Species: Homo sapiens (human)
Strain Ethnicity: Caucasian
Age Stage: 60 years
Gender: Female
Tissue: ovary

Morphology: epithelial
Split Ratio: 1:2 to 1:4
Isoenzyme Analysis: AK-1, 1; ES-D, 1; G6PD, B; GLO-I, 1; PGM1, 1; PGM3, 1
Mycoplasma Test: negative
Tumorigenic: Forms colonies in soft agar, in nude mice inoculated subcutaneously with 10(7) cells
Receptor expressed: androgen receptor, estrogen receptor, progesterone receptor

Incubation Temp(°C): 37°C
Incubation Condition: 5% CO2
Culture Medium: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate and supplemented with 0.01 mg/ml bovine insulin, 80%; fetal bovine serum, 20%
Growth Property: adherent
SubCulture Procedure: by trypsin-EDTA
Freezed Medium: 93% culture medium+7%DMSO
Medium Renewal: Every 2 to 3 days

Description: NIH:OVCAR-3 is an appropriate model system in which to study drug resistance in ovarian cancer, and the presence of hormone receptors should be useful for the evaluation of hormonal therapy.
Others: STR-PCR profile — D7S820:10 CSF1PO:11, 12 TH01:9, 9.3 D13S317:12 D16S539:12 vWA:17 TPOX:8 Amelogenin:X D5S818:11, 12

Image / Description:   200倍視野下低細胞密度
  200倍視野下高細胞密度
  100倍視野下 高細胞密度
  200倍視野下 高細胞密度
 

Biosafety Level: 1
Reference: Cancer Res. 43: 5379-5389, 1983, J. Clin. Endocrinol. Metab. 59: 561-563, 1984.
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