Cell Line

BCRC Number: 60549
Name: MDA-MB-361
Type: adenocarcinoma; mammary gland; breast, from metastatic site: brain
Species: Homo sapiens/human
Age Stage: 40 years
Gender: Female
Tissue: breast

Morphology: epithelial
Split Ratio: 1:2 to 1:6
Isoenzyme Analysis: AK-1, 1; ES-D, 1; G6PD, B; GLO-I, 2; Me-2, 1; PGM1, 1; PGM3, 1-2
Mycoplasma Test: negative
Cytogenetics: modal number. = 56; range = 54 to 61

Incubation Temp(°C): 37°C
Incubation Condition: 0 % CO2
Culture Medium: 80% Leibovitz's L-15 medium with 2 mM L-glutamine+20% fetal bovine serum
Growth Property: loosely adherent
SubCulture Procedure: by trypsin-EDTA
Freezed Medium: 93% culture medium+7%DMSO
Medium Renewal: Every 2 to 3 days

Description: This line differs from others of the series (MDA-MB-134-VI, MDA-MB-157, MDA-MB-175-VII and MDA-MB-231 ) in karyology and in that it was isolated from a brain metastasis.
Others: The cell line is aneuploid human female, with chromosome counts in the hyperdiploid range. Normal chromosomes N11 and N17 are absent, chromosomes N1, N20, and N21 are weakly represented, and chromosomes N2, N8, N9, and N15 are single. STR-PCR profile — D7S820:9 CSF1PO:12 TH01:9.3 D13S317:11 D16S539:11, 12 vWA:17 TPOX:8, 11 Amelogenin:X D5S818:10, 11

Image / Description:   200倍視野下

Biosafety Level: 1
Reference: Cancer Res. 40: 3118-3129, 1980, In Vitro 12: 331, 1976,Cancer Res. 39: 919-922, 1979, Cancer Res. 54: 2615-2621, 1994.
Frozen Vial for all NTD$ 6000 Order this item

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