Cell Line



BCRC Number: 60547 Add To Bookmark
Name: CTX TNA2
Type: Brain, astrocyte, type 1 phenotype, normal
Species: Rattus norvegicus/rat
Strain Ethnicity: Sprague-Dawley
Age Stage: neonate
Gender: unknown
Tissue: cortex

Morphology: fibroblast
Split Ratio: 1:2 to 1:8
Mycoplasma Test: negative
Tumorigenic: No, The cells were not tumorigenic in immunosuppressed mice, but did form colonies in semisolid medium.

Incubation Temp(°C): 37°C
Incubation Condition: 5% CO2
Culture Medium: 90% Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose + 10% fetal bovine serum
Growth Property: adherent
SubCulture Procedure: by trypsin-EDTA
Freezed Medium: 93% culture medium+7 %DMSO
Medium Renewal: Every 2 to 3 days

Description: The CTX TNA2 cell line was established from primary cultures of type 1 astrocytes from brain frontal cortex tissue of 1 day old rats.The cultures were transfected 3 days after initial plating with a DNA construct containing the oncogenic early region of SV40 under the transcriptional control of the human GFAP promoter (pGFA-SV-Tt).
Product Character: alpha 2 macroglobulin; transferrin

Image / Description:   200倍高細胞密度
  200倍低細胞密度
 

Biosafety Level: 1
Shipping/Field/Price:
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