Cell Line

BCRC Number: 60545 Add To Bookmark
Name: 22Rv1
Type: Prostate carcinoma
Species: Homo sapiens human
Gender: Male

Morphology: epithelial
Split Ratio: 1:3-1:6
Mycoplasma Test: negative
Tumorigenic: forms tumors in nude mice
Receptor expressed: androgen receptor

Incubation Temp(°C): 37°C
Incubation Condition: 5%CO2
Culture Medium: 90% RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate+10% fetal bovine serum
Growth Property: adherent
SubCulture Procedure: by trypsin-EDTA
Freezed Medium: 93% culture medium+7% DMSO
Medium Renewal: Every 2 to 3 days

Description: The cell line expresses prostate specific antigen (PSA). Growth is weakly stimulated by dihydroxytestosterone and lysates are immunoreactive with androgen receptor antibody by Western blot analysis.Growth is stimulated by epidermal growth factor (EGF) but is not inhibited by transforming growth factor beta-1 (TGF beta- 1).
Others: STR-PCR profile — D7S820:9, 10, 11 CSF1PO:10, 11 TH01:6, 9.3 D13S317:9, 12 D16S539:12 vWA:15, 21 TPOX:8 Amelogenin:X, Y D5S818:11, 13
Product Character: prostate specific antigen

Image / Description:   200倍視野下

Biosafety Level: 2
Reference: In Vitro Cell. Dev. Biol. Anim. 35: 403-409, 1999.
Frozen Vial for all NTD$ 6000 Order this item

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