Cell Line



BCRC Number: 60543 Add To Bookmark
Name: MJ
Type: peripheral blood, T lymphocyte, cutaneous T cell lymphoma; mycosis fungoides
Species: Homo sapiens human
Age Stage: 50 years
Gender: Male
Tissue: peripheral blood

Morphology: lymphoblast
Split Ratio: 1:2 to 1:3
Mycoplasma Test: negative
Receptor expressed: interleukin 2 (IL-2)

Incubation Temp(°C): 37°C
Incubation Condition: 5% CO2
Culture Medium: Iscove's modified Dulbecco's medium, 80%; fetal bovine serum, 20%
Growth Property: suspension
SubCulture Procedure: Pellet cells by centrigation, save one third of the spent medium and to two volumes of fresh medium
Freezed Medium: 93% culture medium+7% DMSO
Seeding Density: Establish new cultures at about 2 X 10(5) cells/ml.
Medium Renewal: Twice per week

Description: The cells express a cell surface glycoprotein of 61000 daltons (45000 dalton when not glycosylated) that is characteristic of HTLV infection
Others: STR-PCR profile — D7S820:8,11 CSF1PO:11,12 TH01:6,7 D13S317:12,14 D16S539:11,12 vWA:15,17 TPOX:8,11 Amelogenin:X,Y D5S818:11,13

Image / Description:   100倍視野下
 

Biosafety Level: 2
Reference: US Patent 4,743,678 dated May 10 1988Science 219: 856-859, 1983.
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