Cell Line



BCRC Number: 60539 Add To Bookmark
Name: HCT-15
Type: colorectal adenocarcinoma
Species: Homo sapiens (human)
Gender: Male
Tissue: colon

Morphology: epithelial
Split Ratio: 1:2 to 1:10
Mycoplasma Test: negative
Tumorigenic: yes, in nude mice

Incubation Temp(°C): 37°C
Incubation Condition: 5%CO2
Culture Medium: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate, 90%; fetal bovine serum, 10%
Growth Property: adherent
SubCulture Procedure: by trypsin-EDTA
Freezed Medium: 93% culture medium+7% DMSO
Medium Renewal: every 2 to 3 days

Others: STR-PCR profile — D7S820:10, 12 CSF1PO:12 TH01:7, 9.3 D13S317:8, 11 D16S539:12, 13 vWA:18, 19 TPOX:8, 11 Amelogenin:X, Y D5S818:13
Product Character: carcinoembryonic antigen(CEA);keratin

Image / Description:   200倍視野下
 

Biosafety Level: 1
Reference: Cancer Genet. Cytogenet. 81: 103-108, 1995. Int. J. Cancer 41: 287-296, 1988.Cancer Res. 39: 1020-1025, 1979. Cancer Res. 58: 95-101, 1998.Cancer Genet. Cytogenet. 107:76-79,1998.
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