Cell Line



BCRC Number: 60536
Name: LS123
Type: colorectal adenocarcinoma
Species: Homo sapiens (human)
Age Stage: 65 years
Gender: Female
Tissue: colon

Morphology: epithelial
Split Ratio: 1:4 to 1:8
Mycoplasma Test: negative

Incubation Temp(°C): 37°C
Incubation Condition: 5%CO2
Culture Medium: 90% Minimum essential medium (Eagle) with 2 mM L-glutamine and Earle's BSS adjusted to contain 1.5 g/L sodium bicarbonate, 0.1 mM non-essential amino acids, and 1.0 mM sodium pyruvate+10% fetal bovine serum
Growth Property: adherent
SubCulture Procedure: by 0.25% trypsin-EDTA
Freezed Medium: 93 %culture medium+ 7% DMSO
Medium Renewal: every 2 to 3 days

Description: LS123 was from the second in a series of three primary colonic tumors resected from a 65 year old Caucasian female.The cell line is aneuploid, releases small amounts of carcinoembryonic antigen, fails to grow progressively in nude mice, and forms colonies only in enriched semisolid medium developed for tumor stem cells.
Others: STR-PCR profile — D7S820:9 CSF1PO:10, 11 TH01:7, 8 D13S317:9, 13 D16S539:13 vWA:16 TPOX:9 Amelogenin:X D5S818:11
Product Character: carcinoembryonic antigen (CEA), 9 ng/ml per 10 exp6 cells per 10 days; mucin

Image / Description:   200倍視野下高細胞密度
 

Biosafety Level: 1
Reference: In Vitro 19: 99-107, 1983
Shipping/Field/Price:
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