Cell Line

BCRC Number: 60522
Name: HuT78
Type: cutaneous T lymphocyte,human
Species: Homo sapiens (human)
Age Stage: 53 years
Gender: Male
Tissue: lymphoma

Morphology: lymphoblast
Split Ratio: maintain cell density at less than 1 X 10 exp6 cells/ml.
Mycoplasma Test: Negative
Tumorigenic: yes, in nude mice when injected intracranially

Incubation Temp(°C): 37°C
Incubation Condition: 5%CO2
Culture Medium: Iscove's modified Dulbecco's medium with 4mM L-glutamine and 1.5g/L sodium bicarbonate, 80%(不可使用RPMI), fetal bovine serum, 20%
Growth Property: suspension
SubCulture Procedure: Cultures can be maintained by addition of fresh medium or replacement of medium.
Freezed Medium: 93% culture medium+7%DMSO
Seeding Density: 2 X 10 exp5 viable cells/ml.
Medium Renewal: every 2 to 3 days

Description: The line has properties of a mature human T cell with helper/inducer activity. It releases IL-2 and has receptors for IL-2.
Others: STR-PCR profile — D7S820:8,11 CSF1PO:11,12 TH01:8,9 D13S317:8,12 D16S539:11,12 vWA:14,15 TPOX:8,9 Amelogenin:X,Y D5S818:11,12
Product Character: interleukin2 (IL-2); tumor necrosis factor alpha(TNF alpha)

Image / Description:   200倍視野下

Biosafety Level: 1
Reference: 1. J. Exp. Med. 154: 1403-1418, 1981.2.AIDS Res. Hum. Retroviruses 5: 253-255, 1989.3. Proc. Natl. Acad. Sci. USA 93: 14188-14192, 1996.
Frozen Vial for all NTD$ 6000 Order this item

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