Cell Line



BCRC Number: 60519
Name: Hs683
Type: glioma
Species: Homo sapiens (human)
Strain Ethnicity: Caucasian
Age Stage: 76 years
Gender: Male
Tissue: brain

Morphology: fibroblast
Split Ratio: 1:4
Mycoplasma Test: negative
Tumorigenic: No, in immunosuppressed mice; yes, in semisold medium

Incubation Temp(°C): 37°C
Incubation Condition: 5%CO2
Culture Medium: Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose,90%;fetal bovine serum,10%.
Growth Property: Adherent
SubCulture Procedure: trypsin-EDTA
Freezed Medium: 93% culture medium + 7% DMSO
Medium Renewal: every 2 to 3 days

Description: Hs683 cell line was isolated from explant cultures of a glioma taken from the left temporal lobe of a 76 year old male Caucasian. Microvilli but not desomosomes were observed.
Others: STR-PCR profile — D7S820:11 CSF1PO:9,13 TH01:6,8 D13S317:8,12 D16S539:9,10 vWA:18,20 TPOX:8,11 Amelogenin:X,Y D5S818:11,12

Image / Description:   100倍視野下
  200倍視野下
 

Biosafety Level: 1
Reference: 1.J.Natl. Cancer Inst, 56: 843-849, 19762.Cancer Res.52:2523-2529,1992.3.Cancer Inst.58:1455-1463,1977.4. J. Biol. Chem.271: 22428-22433,1996.
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