Cell Line



BCRC Number: 60511
Name: CA46
Type: B lymphocyte; Burkitt’s lymphoma, IgM; Kappa light chain
Species: Homo sapiens (human)
Gender: Male
Tissue: B lymphocyte; Burkitt’s lymphoma

Morphology: lymphoblast
Split Ratio: maintain cell density between 105 to 106 cells/ml
Isoenzyme Analysis: IgM; kappa light chain
Mycoplasma Test: negative
Doubling time: 16 hours
Receptor expressed: complement(12% of the cells)

Incubation Temp(°C): 37°C
Incubation Condition: 5% CO2
Culture Medium: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate, 80%;fetal bovine serum,20%.
Growth Property: Suspension
SubCulture Procedure: Addition or replacement of fresh medium
Freezed Medium: 93% culture medium + 7% DMSO
Seeding Density: 2-3 x 105 cells/ml
Medium Renewal: every 2 to 3 days

Description: The cells are EBNA negative and are negative for Fc receptors. Approximately 12% of the cells express commplement receptors.
Others: STR-PCR profile — D7S820:11,12 CSF1PO:10,12 TH01:7,9 D13S317:8,12 D16S539:11,12 vWA:15,16 TPOX:8,9 Amelogenin:X D5S818:13
Product Character: immunoglobin(surface and secreted)

Image / Description:   200倍視野下
 

Biosafety Level: 1
Reference: J. Immunol. 129: 1336-1342, 1982.,J. Natl. Cancer Inst. 64: 465-476, 1980.,J. Natl. Cancer Inst. 64: 477-483, 1980.
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