Cell Line



BCRC Number: 60505
Name: kasumi-1
Type: human acute myeloblastic leukemia
Species: Homo sapiens (human)
Strain Ethnicity: Japanese
Age Stage: 7 years
Gender: Male
Tissue: peripheral blood; myeloblast; acute myeloblastic leukemia

Morphology: myeloblast
Split Ratio: maintain cell density between 3 x 10 5 and 3 x 10 6 viable cells/ml
Mycoplasma Test: negative
Cytogenetics: t(8:21) (q22;q22)
Doubling time: 40 to 48 hours

Incubation Temp(°C): 37°C
Incubation Condition: 5% CO2
Culture Medium: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate,80%;fetal bovine serum,20%.
Growth Property: suspension
SubCulture Procedure: addition of fresh medium or replacement of fresh medium
Freezed Medium: 93% culture medium + 7% DMSO
Seeding Density: 3 x 105 viable cells/ml
Medium Renewal: every 2 to 3 days (depending on cell density)

Description: The cell line was established from the peripheral blood of an acute myeloid leukemia (AML) patient. This is a leukemic cell line with an 8;21 chromosome translocation. This translocation juxtaposes the AML1 with ETO (or MTG8) gene, giving rise to the fusion gene AML1-ETO(also known as AML-MTG or RUNX1-CBF2T1), hence the cells produce chimeric AML-ETO protein.This protein down-regulate CEBPA mRNA, protein and DNA binding activity,which is crucial for the differentiation of granulocytes. The cells are positive for myeloperoxidase showing a morphology of myeloid maturation.
Others: 1.該株細胞增值速度緩慢.故冷凍保存細胞數目不宜過低且培養時稀釋倍數不宜過高 2.STR-PCR profile — D7S820:8, 11 CSF1PO:10, 12 TH01:6, 9 D13S317:11, 13 D16S539:9, 12 vWA:14 TPOX:8, 9 Amelogenin:X D5S818:9, 11
Product Character: myeloperoxidase

Image / Description:   100倍視野下低細胞密度
 

Biosafety Level: 1
Reference: Blood 77: 2031-2036, 1991; EMBO J. 12: 2715-2721, 1993; Natl. Acad. Sci. USA 88: 10431-10434, 1991; Blood 92: 2441-2449, 1998; Blood 82: 1270-1276, 1993; Nat. Med. 7: 444-451, 2001
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