Cell Line



BCRC Number: 60504 Add To Bookmark
Name: WI-38 VA-13 subline 2RA
Type: human lung, SV40 transformed
Species: Homo sapiens (human)
Strain Ethnicity: Caucasian
Age Stage: 3 months gestation
Gender: unknown
Tissue: lung; SV40 transformed

Morphology: epithelial
Split Ratio: 1:2 to 1:10
Mycoplasma Test: negative
Cytogenetics: modal number = 76; range = 45 to 89.
Virus susceptibility: Poliovirus 2; Herpes simplex virus; Vesicular stomatitis Indiana virus

Incubation Temp(°C): 37°C
Incubation Condition: 5% CO2
Culture Medium: Minimum essential medium(Eagle) with 2 mM L-glutamine and Earle’s BSS adjusted to contain 1.5 g/L sodium bicarbonate, 0.1 mM non-essential amino acids, and 1.0 mM sodium pyruvate, 90%;fetal bovine serum,10%.
Growth Property: adherent
SubCulture Procedure: trypsin-EDTA
Freezed Medium: 93% culture medium + 7% DMSO
Medium Renewal: every 2 to 3 days

Others: STR-PCR profile — D7S820:9, 11 CSF1PO:10, 12 TH01:9.3 D13S317:11 D16S539:11, 12 vWA:19, 20 TPOX:8 Amelogenin:X D5S818:10

Image / Description:   200倍視野下低細胞密度
  200倍視野下高細胞密度
 

Biosafety Level: 1
Reference: Ann. Med. Exp. Biol. Fenn. 44: 242-254, 1966; J. Natl. Cancer Inst. 32: 917-937, 1964; J. Virol. 70: 8422-8430, 1996
Shipping/Field/Price:
Frozen Vial for all NTD$ 6000 Order this item

Bioresource Collection and Research Center

331, Shih-Pin Rd., Hsinchu 300193, Taiwan

Phone: +886-3-5223191

E-mail: bcrcweb@firdi.org.tw

Copyright © 2018.BCRC All rights reserved.

web maintainance: +886-3-5223191 ext 572

Privacy Policy        Cookie