Cell Line

BCRC Number: 60502
Name: KU812
Type: human chronic myelogenous leukemia
Species: Homo sapiens (human)
Strain Ethnicity: Japanese
Age Stage: 38 years
Gender: Male
Tissue: peripheral blood; basophil; chronic myelogenous leukemia

Morphology: myeloblast
Split Ratio: Maintain cell density between 3 x 105 and 3 x 106 cells/ml; Do not allow the cell concentration to exceed 3 x 106 cells/ml.
Mycoplasma Test: negative
Cytogenetics: 58, XY, t(9;22)(q34;q11), i(17q), +1, +4, +6, +6, +8, +der(9)t(9;22), + der(9)t(9;22), +der(11)q(11), +14, +15, +19, +21, +21, -9

Incubation Temp(°C): 37°C
Incubation Condition: 5% CO2
Culture Medium: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate,90%;fetal bovine serum,10%.
Growth Property: suspension
SubCulture Procedure: addition or replacement of fresh medium
Freezed Medium: 93% culture medium + 7% DMSO
Seeding Density: 2 x 105 cells/ml
Medium Renewal: every 2 to 3 days

Description: The KU812 cell line was established from the peripheral blood of a patient in blast crisis of chronic myelogenous leukemia. The cells contain at least one Ph1 (Philadelphia) chromosome. The cell line has some characteristics of basophilic leukocytes (Fc receptors, basophilic granules, histamine production), and are negative for lymphoid markers.)
Others: STR-PCR profile — D7S820:12 CSF1PO:12,13 TH01:9 D13S317:8 D16S539:9 vWA:15 TPOX:12 Amelogenin:X,Y D5S818:10
Product Character: transforming growth factor beta

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Biosafety Level: 1
Reference: Leuk. Res. 9: 381-390, 1985; Biochem. 104: 162-164, 1988; Nippon Ketsueki Gakkai Zasshi 52: 1423-1432, 1989; Eur. J. Immunol. 22: 2025-2032, 1992
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