Cell Line



BCRC Number: 60502
Name: KU812
Type: human chronic myelogenous leukemia
Species: Homo sapiens (human)
Strain Ethnicity: Japanese
Age Stage: 38 years
Gender: Male
Tissue: peripheral blood; basophil; chronic myelogenous leukemia

Morphology: myeloblast
Split Ratio: Maintain cell density between 3 x 105 and 3 x 106 cells/ml; Do not allow the cell concentration to exceed 3 x 106 cells/ml.
Mycoplasma Test: negative
Cytogenetics: 58, XY, t(9;22)(q34;q11), i(17q), +1, +4, +6, +6, +8, +der(9)t(9;22), + der(9)t(9;22), +der(11)q(11), +14, +15, +19, +21, +21, -9

Incubation Temp(°C): 37°C
Incubation Condition: 5% CO2
Culture Medium: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate,90%;fetal bovine serum,10%.
Growth Property: suspension
SubCulture Procedure: addition or replacement of fresh medium
Freezed Medium: 93% culture medium + 7% DMSO
Seeding Density: 2 x 105 cells/ml
Medium Renewal: every 2 to 3 days

Others: STR-PCR profile — D7S820:12 CSF1PO:12,13 TH01:9 D13S317:8 D16S539:9 vWA:15 TPOX:12 Amelogenin:X,Y D5S818:10
Product Character: transforming growth factor beta

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Biosafety Level: 1
Reference: Leuk. Res. 9: 381-390, 1985; Biochem. 104: 162-164, 1988; Nippon Ketsueki Gakkai Zasshi 52: 1423-1432, 1989; Eur. J. Immunol. 22: 2025-2032, 1992
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