Cell Line

BCRC Number: 60500 Add To Bookmark
Name: EM9
Type: Chinese hamster ovary
Species: Cricetulus griseus (hamster, Chinese)
Gender: Female
Tissue: ovary

Morphology: epithelial
Split Ratio: 1:4 to 1:12
Mycoplasma Test: negative

Incubation Temp(°C): 37°C
Incubation Condition: 5% CO2
Culture Medium: Alpha minimum essential medium without ribonucleosides and deoxyribonucleosides.90% ;fetal bovine serum,10%, with sodium bicarbonate 1.5g/L
Growth Property: mixed: adherent and suspension
SubCulture Procedure: trypsin-EDTA(for attached cells)
Freezed Medium: 93% culture medium + 7% DMSO
Medium Renewal: every 2 to 3 days

Description: This line is a derivative of the CHO-K1 cell line. EM9 is a repair deficient mutant derived from AA8 . The line was selected for enhanced sensitivity to ethylmethanesulfonate (EMS). The line is defective in single strand break repair, has a 10 fold higher.

Image / Description:   100倍視野下高細胞密度

Biosafety Level: 1
Reference: Mutat. Res. 95: 427-440, 1982; Somatic Cell Genet. 6: 391-405, 1980
Frozen Vial for all NTD$ 6000 Order this item

Bioresource Collection and Research Center

331, Shih-Pin Rd., Hsinchu 300193, Taiwan

Phone: +886-3-5223191

E-mail: bcrcweb@firdi.org.tw

Copyright © 2018.BCRC All rights reserved.

web maintainance: +886-3-5223191 ext 572

Privacy Policy        Cookie