Cell Line



BCRC Number: 60494
Name: AsPC-1
Type: human pancreatic adenocarcinoma, from ascites
Species: Homo sapiens (human)
Strain Ethnicity: Caucasian
Age Stage: 62 years
Gender: Female
Tissue: pancreas; metastatic site: ascites adenocarcinoma

Morphology: epithelial
Split Ratio: 1:3 to 1:6
Mycoplasma Test: negative

Incubation Temp(°C): 37°C
Incubation Condition: 5% CO2
Culture Medium: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate,90%;fetal bovine serum,10%.
Growth Property: adherent
SubCulture Procedure: trypsin-EDTA
Freezed Medium: 93% culture medium + 7% DMSO
Medium Renewal: every 2 to 3 days

Description: The line was derived from nude mouse xenografts initiated with cells from the ascites of a patient with cancer of the pancreas.
Others: STR-PCR profile — D7S820:12, 13 CSF1PO:10, 13 TH01:7, 9.3 D13S317:9, 12 D16S539:11 vWA:17 TPOX:8, 10 Amelogenin:X D5S818:12
Product Character: carcinoembryonic antigen (CEA); human pancreas associated antigen; human pancreas specific antigen; mucin

Image / Description:   100倍視野下低細胞密度
  100倍視野下高細胞密度
 

Biosafety Level: 1
Reference: In Vitro 18: 24-34, 1982; . J. Natl. Cancer Inst. 67: 563-569, 1981; Clin. Lab. Med. 2: 567-578, 1982
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