Cell Line

BCRC Number: 60492 Add To Bookmark
Name: PC 61.5.3
Type: mouse hybridoma, anti mouse IL-2 receptor (CD25)
Species: Rattus norvegicus (B cell); Mus musculus (myeloma) (rat (B cell); mouse (myeloma))
Gender: unknown
Tissue: B lymphocyte; hybridoma

Morphology: lymphoblast
Split Ratio: maintain cell density between 1 x 105 and 5 x 105 cells/ml
Mycoplasma Test: negative

Incubation Temp(°C): 37°C
Incubation Condition: 5% CO2
Culture Medium: Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose and supplemented with 0.05 mM 2-mercaptoethanol,90%;fetal bovine serum,10%.
Growth Property: suspension
SubCulture Procedure: addition or replacement of fresh medium
Freezed Medium: 93% culture medium + 7% DMSO
Seeding Density: 1 x 105 cells/ml
Medium Renewal: every 2 to 4 days (depending on cell density)

Description: Animals were immunized with the B6.1 mouse cytotoxic T cell line. Spleen cells were fused with P3X63Ag8.653 myeloma cells. The antibody blocks IL-2 binding and IL-2 dependent growth stimulation, and immunoprecipitates IL-2 receptors
Product Character: immunoglobulin; monoclonal antibody; against mouse interleukin-2 (interleukin 2, IL-2) receptor (CD25); isotype: IgG1

Image / Description:   200倍視野下高細胞密度

Biosafety Level: 1
Reference: J. Cell. Physiol. 112: 403-410, 1982; J. Exp. Med. 160: 1170-1183, 1984
Frozen Vial for all NTD$ 6000 Order this item

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