Cell Line



BCRC Number: 60491 Add To Bookmark
Name: JAR
Type: human choriocarcinoma
Species: Homo sapiens (human)
Strain Ethnicity: Caucasian
Age Stage: fetus
Gender: Male
Tissue: placenta; choriocarcinoma

Morphology: epithelial
Split Ratio: 1:3 to 1:8
Mycoplasma Test: negative
Cytogenetics: pseudotriploid with complex karyotype

Incubation Temp(°C): 37°C
Incubation Condition: 5% CO2
Culture Medium: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate,90%;fetal bovine serum,10%.
Growth Property: adherent
SubCulture Procedure: typsin-EDTA
Freezed Medium: 93% culture medium + 7% DMSO
Medium Renewal: every 3 to 4 days

Description: the line was established directly from a trophoblastic tumor of the placenta; it produces estrogen, progesterone, gonadotropin and lactogen in culture.
Others: STR-PCR profile — D7S820:10, 11 CSF1PO:7, 10 TH01:6, 7 D13S317:11 D16S539:9, 10 vWA:16, 18 TPOX:8, 11 Amelogenin:X, Y D5S818:10, 11
Product Character: estrogen; progesterone; human chorionic gonadotropin (hCG); human chorionic somatomammotropin (placental lactogen); hCG production averages 22.5 ng/ml after reculturing

Image / Description:   200倍視野下高細胞密度
 

Biosafety Level: 1
Reference: In Vitro 6: 398-399, 1971
Shipping/Field/Price:
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