Cell Line



BCRC Number: 60489 Add To Bookmark
Name: LADMAC
Type: mouse bone marrow, producing colony stimulating factor-1
Species: Mus musculus (mouse)
Strain Ethnicity: C3H/HeJ
Age Stage: adult
Gender: unknown
Tissue: bone marrow

Morphology: lymphoblast
Split Ratio: maintain cell density between 1 x 105 and 1 x 106 viable cells/ml
Mycoplasma Test: negative
Tumorigenic: Yes, the cells are tumorigenic in nu+, nu+ mice but not in syngenic mice.

Incubation Temp(°C): 37°C
Incubation Condition: 5% CO2
Culture Medium: Minimum essential medium(Eagle) with 2 mM L-glutamine and Earle’s BSS adjusted to contain 1.5 g/L sodium bicarbonate, 0.1 mM non-essential amino acids, and 1.0 mM sodium pyruvate,90%;fetal bovine serum,10%.
Growth Property: suspension, but with many adherent cells
SubCulture Procedure: addtion or replacement of fresh medium; attached cells may be subcultured by tapping the sides of the flask until cells are dispersed
Freezed Medium: 93% culture medium + 7% DMSO
Seeding Density: 1 to 2 x 105 viable cells/ml
Medium Renewal: every 2 to 3 days (depending on cell density)

Description: 'LADMAC is a transformed cell line derived by transfecting mouse bone marrow cells highly enriched for macrophage progenitors with cloned human cellular myc-homologous sequences covalently attached to pBR325 (pR myc). LADMAC cells secrete the growth factor colony stimulating factor 1 (CSF-1). CSF-1 is capable of supporting the in vitro proliferation of mouse bone marrow macrophages. The Pannell-Milstein roller bottle apparatus may be used to produce high concentrations of CSF-1(PubMed: 7769247). This cell line is used to produce LADMAC conditioned medium. It will support the growth of the macrophage cell lines EOC 2, EOC 13.31, EOC 20, I-11.15 and I-13.35. Conditioned medium prepare: 1. Allow cells to become confluent. 2. After 5 to 7 days, collect supernate, centrifuge at 125 xg for 5 to 10 minutes. 3. Filter (200 nM filter). 4. Store aliguots at -20℃.
Product Character: colony stimulating factor-1 (CSF-1)

Image / Description:   100倍視野下高細胞密度
  100倍視野下高細胞密度
  100倍視野下
  200倍視野下
  100倍視野下
  200倍視野下
 

Biosafety Level: 1
Reference: J. Cell. Physiol. 125: 403-412, 1985; J. Immunol. Methods 182: 73-79, 1995
Shipping/Field/Price:
Frozen Vial for all NTD$ 7000 Order this item

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