Cell Line



BCRC Number: 60482 Add To Bookmark
Name: NCTC clone 1469 (derivative of NCTC 721)
Type: mouse liver
Species: Mus musculus/(mouse)
Strain Ethnicity: C3H/An
Age Stage: newborn
Gender: Male
Tissue: liver; normal

Morphology: epithelial
Split Ratio: 1:2 to 1:4
Mycoplasma Test: negative
Virus resistance: Poliovirus 1
Virus susceptibility: vesicular stomatitis (Indiana)

Incubation Temp(°C): 37°C
Incubation Condition: 5% CO2
Culture Medium: Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose,90%;horse serum,10%.
Growth Property: adherent
SubCulture Procedure: 'by scraping; or by trypsin-EDTA; NOTE: The culture medium in heavy cultures may be turbid in appearance. It is characteristic of this line to shed viable cells into the medium, thus rendering the medium turbid. The shed cells are viable and may be used to initiate new cultures.
Freezed Medium: 93% culture medium + 7% DMSO
Medium Renewal: every 2 to 3 days


Image / Description:   200倍視野下高細胞密度
 

Biosafety Level: 1
Reference: J. Natl. Cancer Inst. 18: 701, 1957; J. Natl. Cancer Inst. 12: 1245, 1952; J. Natl. Cancer Inst. 23: 823, 1959; J. Natl. Cancer Inst. 27: 29, 1961; Cancer Res. 12: 261, 1958; Fed. Proc. 17: 967, 1958; Natl. Cancer Inst. Monogr. 7: 147, 1962
Shipping/Field/Price:
Frozen Vial for all NTD$ 6000 Order this item

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