Cell Line

BCRC Number: 60474
Name: GCT
Type: human fibrous histiocytoma
Species: Homo sapiens (human)
Age Stage: 29 years
Gender: Male
Tissue: metastatic site: lung fibrous histiocytoma

Split Ratio: 1:2 to 1:4
Mycoplasma Test: negative

Incubation Temp(°C): 37°C
Incubation Condition: 5% CO2
Culture Medium: McCoy's 5 a medium with 1.5 mM L-glutamine,90% ;fetal bovine serum,10%
Growth Property: adherent
SubCulture Procedure: trypsin-EDTA
Freezed Medium: 93% culture medium + 7% DMSO
Medium Renewal: every 2 to 3 days

Description: 'established from fragments of metatstatic lung tiusse with malignant fibrous histiocytoma; this cell line spontaneously elaborates colony-stimulating activity (CSA) for human granulocyte progenitors and erythroid-enhancing activity (EAA) for erythroid precursors into serum-free culture medium; the conditioned medium from this cell line can be used as a source of prostaglandin E and plasminogen activator; the line itself has surface antignes compatible with monocytes and fibroblasts.
Others: STR-PCR profile — D7S820:11,12 CSF1PO:12 TH01:8,9.3 D13S317:11,12 D16S539:9 vWA:16,18 TPOX:8,9 Amelogenin:X D5S818:13,15
Product Character: colony stimulating activity (CSA); erythroid enhancing activity (EEA); prostaglandin E; plasminogen activator

Image / Description:  

Biosafety Level: 1
Patent Statement: US Patent 4,135,975 dated Jan 23 1979
Reference: Blood 51: 507-519, 1978; Blood 56: 717-727, 1980; Blood 58: 1148-1154, 1981; . Supramol. Struct. 13: 199-209, 1980
Frozen Vial for all NTD$ 6000 Order this item

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