Cell Line



BCRC Number: 60470 Add To Bookmark
Name: PG-4
Type: Cat brain astrocyte, Moloney murine sarcoma virus (Mo-MSV) transformed
Species: Felis catus (cat)
Age Stage: embryo
Gender: unknown
Tissue: brain; normal; astrocyte; Moloney murine sarcoma virus (Mo-MSV)transformed

Morphology: glial,astrocyte
Split Ratio: 1:5
Mycoplasma Test: negative
Virus susceptibility: xenotropic murine leukemia virus; amphotropic murine leukemia virus; mink cell focus forming (MCF) virus; RD-114 virus; feline leukemia virus (FeLV) A, B, C; baboon endogenous virus (BaEV); macaque virus; simian sarcoma associated virus (SSAV); feline i

Incubation Temp(°C): 37°C
Incubation Condition: 5% CO2
Culture Medium: 90% McCoy's 5a medium with 1.5 mM L-glutamien + 10% fetal bovine serum
Growth Property: adherent
SubCulture Procedure: trypsin-EDTA
Freezed Medium: 93% culture medium + 7% DMSO
Medium Renewal: every 2 to 3 days

Description: the line was derived from the G355 cell line by transformation with Mo-MSV; it is resistant to infection and focus formation by ecotropic MuLV; the cells are useful for detection and assay of various C type retroviruses; PG-4 is one of the cell lines that

Image / Description:   100倍視野下高細胞密度
 

Biosafety Level: 1
Reference: J. Virol. 53: 827-833, 1985; Virology 123: 139-151, 1982
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