Cell Line

BCRC Number: 60466
Name: CG1253
Type: blood, mother of dihydropteridine reductase(DHPR) deficient hyperphenylalaninemia (MIM 261630) patient CG1254, clinical normal
Species: homo sapiens
Age Stage: 39 years
Gender: Female
Tissue: blood

Morphology: lymphoblast
Isoenzyme Analysis: NP, G6PD, MD, LD
Mycoplasma Test: negative

Incubation Temp(°C): 37°C
Incubation Condition: 5%CO2
Culture Medium: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate,85% ;heat-inactivated fetal bovine serum,15%.
Growth Property: suspension
SubCulture Procedure: dilute by fresh medium
Freezed Medium: 93% culture medium+7% DMSO
Medium Renewal: every 2 to 3 days

Deposit History: CG1253 is the mother of a DHPR (dihydropteridine reductase; gene synbol: DHPR)deficient family, The mutation of this family is c.697 A>Cunknown mutation.
Depositor: 蕭廣仁
Deposit Date: 2003-03-13
Deposit location: 台北榮民總醫院

Image / Description:   100倍視野下

Biosafety Level: 1
Use Restriction: The cells are distributed for research purpose only.
Reference: 1.J. Biomed Lab Sci 1995;7:A23 2.Berlin: Walter de Gruyter 1990;402-7
Frozen Vial for all NTD$ 6000 Order this item

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