Cell Line



BCRC Number: 60456
Name: 32D Clone 3
Type: lymphoblast
Species: Mus musculus (mouse)
Gender: unknown
Tissue: bone marrow

Morphology: lymphoblast
Isoenzyme Analysis: G6PD, MD, LD.

Incubation Temp(°C): 37°C
Incubation Condition: 5%CO2
Culture Medium: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate supplemented with: 10% heat-inactivated fetal bovine serum 10% mouse Interleukin-3 culture supplement (available from Becton Dickinson Cat. No. 354040, formerly Collaborative Research Cat. No. 40040)
Growth Property: mixed, adherent and suspension
SubCulture Procedure: Cultures can be maintained by the addition of fresh medium or replacement of medium.Scrape off the attached cells and transfer along with the floating cells into new flasks
Freezed Medium: 93% culture medium+7% DMSO
Seeding Density: 2 X 10 5 viable cells/ml.
Medium Renewal: Every 2 to 3 days


Image / Description:   200倍視野下
 

Biosafety Level: 1
Use Restriction: This material is cited in a U.S and or other patent or patent application, and may not be used to infringe on the patent claims
Reference: 1.Leuk. Res. 21: 609-618, 1997. 2.Biochem. Pharmacol. 57: 387-396, 1999.
Shipping/Field/Price:
Frozen Vial for all NTD$ 6000 Order this item

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