Cell Line

BCRC Number: 60455 Add To Bookmark
Name: NCI-H441 (H441)
Type: papillary adenocarcinoma
Species: Homo sapiens (human)
Gender: Male
Tissue: lung

Morphology: epithelial
Split Ratio: 1:3 to 1:8
Isoenzyme Analysis: NP, G6PD, MD, LD.
Mycoplasma Test: negative
Doubling time: 58 hours

Incubation Temp(°C): 37°C
Incubation Condition: 5%CO2
Culture Medium: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate, 90%; fetal bovine serum, 10%
Growth Property: adherent
SubCulture Procedure: by 0.25% (w/v) Trypsin- 0.53 mM EDTA
Freezed Medium: 93% culture medium+7% DMSO
Medium Renewal: 2 to 3 times per week

Description: The cell line expresses mRNA and protein of the major surfactant apoprotein (SP-A).Electron microscopy shows multilamellar bodies and cytoplasmic structures resembling clara cell granules.The cells can be cloned in soft agar with or without serum.The line has been used as a transfection host for expression of pulmonary surfactant protein (SP-B).
Others: STR-PCR profile — D7S820:10 CSF1PO:11, 12 TH01:9.3 D13S317:9 D16S539:9, 13 vWA:17 TPOX:8, 10 Amelogenin:X, Y D5S818:11, 12

Image / Description:   200倍視野下低細胞密度

Biosafety Level: 1
Use Restriction: This cells are distributed for research purpose only.
Reference: 1.Oncogene 4: 45-50, 1989.2.Cancer Res. 46: 798-806, 1986. 3.Proc. Natl. Acad. Sci. USA 93: 14030-14035, 1996.
Frozen Vial for all NTD$ 6000 Order this item

Bioresource Collection and Research Center

331, Shih-Pin Rd., Hsinchu 300193, Taiwan

Phone: +886-3-5223191

E-mail: bcrcweb@firdi.org.tw

Copyright © 2018.BCRC All rights reserved.

web maintainance: +886-3-5223191 ext 572

Privacy Policy        Cookie