Cell Line

BCRC Number: 60451
Name: IGEL b4
Type: mouse hybridoma, against 2,4,6-trinitrophenyl (TNP)
Species: Mus musculus (B cell); Mus musculus (myeloma) (mouse (B cell); mouse (myeloma))
Strain Ethnicity: C57BL/6 (B cell)
Gender: unknown
Tissue: hybridoma; B lymphocyte

Morphology: lymphoblast
Split Ratio: maintain cell density between 105 and 106 cells/ml
Isoenzyme Analysis: MD,LD
Mycoplasma Test: negative

Incubation Temp(°C): 37°C
Incubation Condition: 5% CO2
Culture Medium: Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose,90%;fetal bovine serum,10%.
Growth Property: suspension
SubCulture Procedure: addition or replacement of fresh medium
Freezed Medium: 93% culture medium + 7% DMSO
Seeding Density: 1-2 x 105 cells/ml
Medium Renewal: add fresh medium (depending on cell density) every 2-3 days

Description: Animals were immunized with TNP-Asc.
Product Character: immunoglobulin; monoclonal antibody; against 2,4,6-trinitrophenyl (TNP)

Image / Description:   100倍視野下

Biosafety Level: 1
Reference: Eur. J. Immunol. 11: 527-529, 1981
Frozen Vial for all NTD$ 6000 Order this item

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