Cell Line



BCRC Number: 60445 Add To Bookmark
Name: RTG-2
Type: rainbow trout gonadal tissue
Species: Oncorhynchus mykiss (trout, rainbow) deposited as Salmo gairdneri
Gender: From Mixed Male and Female Organisms
Tissue: gonadal tissue; mixed; testis; ovary

Morphology: fibroblast
Split Ratio: culture can be established with as few as 3 x 103 cells/ml
Isoenzyme Analysis: NP,G6PD,MD,LD
Mycoplasma Test: negative
Virus resistance: poliovirus 1
Virus susceptibility: infectious pancreatic necrosis; Egtved virus; eastern equine encephalitis; venezuelan equine encephalitis

Incubation Temp(°C): 22°C
Incubation Condition: 5% CO2
Culture Medium: minimum essential medium(Eagle) with 2 mM L-glutamine and Earle's BSS adjusted to contain 1.5 g/L sodium bicarbonate, 0.1 mM non-essential amino acids, and 1.0 mM sodium pyruvate,90%;fetal bovine serum,10%.
Growth Property: adherent
SubCulture Procedure: trypsin-EDTA
Freezed Medium: 93% culture medium + 7% DMSO
Medium Renewal: once per week

Description: the line was derived in 1960 from pooled male and female gonadal tissues of yearling rainbow trout; it is the first cell line to be established form cold-blooded vetebrates; maximum growth rate occurs at 22C; above 26C the cells do not survive.

Image / Description:   200倍視野下
 

Biosafety Level: 1
Reference: Science 135: 1065, 1962; Proc. Soc. Exp. Biol. Med. 116: 190, 1964
Shipping/Field/Price:
Frozen Vial for all NTD$ 7000 Order this item

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