Cell Line



BCRC Number: 60439
Name: 143 B
Type: human osteosarcoma, tk-
Species: Homo sapiens (human)
Age Stage: 13 years
Gender: unknown
Tissue: bone; osteosarcoma

Morphology: mixed, epithelial and fibroblast
Split Ratio: 1:3 to 1:8
Mycoplasma Test: negative

Incubation Temp(°C): 37°C
Incubation Condition: 5% CO2
Culture Medium: 90% minimum essential medium (Eagle) in Earle's BSS with 0.015 mg/ml 5-bromo-2'-deoxyuridine + 10% fetal bovine serum
Growth Property: adherent
SubCulture Procedure: trypsin-EDTA
Freezed Medium: 93% culture medium + 7% DMSO
Medium Renewal: every 2 to 3 days

Description: the cells are thymidine kinase deficient (TK-) and are resistant to 5-bromo-2' deoxyurine
Others: STR-PCR profile — D7S820:11, 12 CSF1PO:12 TH01:6 D13S317:12 D16S539:10, 13 vWA:18 TPOX:11 Amelogenin:X D5S818:13

Image / Description:   100倍視野下低細胞密度
  100倍視野下高細胞密度
 

Biosafety Level: 1
Shipping/Field/Price:
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