Cell Line

BCRC Number: 60437
Name: U266B1 U266
Type: human IgE myeloma
Species: Homo sapiens (human)
Age Stage: 53 years
Gender: Male
Tissue: myeloma; plasmacytoma; B lymphocyte

Morphology: lymphoblast
Split Ratio: maintain cell density between 105 to 106 cells/ml
Isoenzyme Analysis: NP,G6PD,MD,LD
Mycoplasma Test: Negative

Incubation Temp(°C): 37°C
Culture Medium: RPMI 1640 medium with 2mM L-glutamine adjusted to contain 1.5g/L sodium bicarbonate,,85%;fetal bovine serum,15%.
Growth Property: suspension
SubCulture Procedure: Addition or replacement of fresh medium
Freezed Medium: 93% culture medium + 7% DMSO
Seeding Density: 3 x 105 cells ml
Medium Renewal: Every 2 to 3 days

Description: established from the peripheral blood of a patient with an IgE myeloma (epsilon2, lambda2); the cells secrete IgE; the cells have been reported to produce human IL-6
Others: STR-PCR profile — D7S820:11, 12 CSF1PO:12, 13 TH01:5, 7 D13S317:12 D16S539:10 vWA:17 TPOX:8 Amelogenin:X, Y D5S818:11, 12
Product Character: immunoglobulin; monoclonal antibody (isotype: IgE, lambda light chain); interleukin 6 (interleukin-6, IL-6)

Image / Description:   200倍視野下

Biosafety Level: 1
Reference: Clin. Exp. Immunol. 7: 477-489, 1970; Nature 332: 83-85, 1988
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