Cell Line

BCRC Number: 60436 Add To Bookmark
Name: MCF-7
Type: human breast adenocarcinoma
Species: Homo sapiens (human)
Strain Ethnicity: Caucasian
Age Stage: 69 years
Gender: Female
Tissue: breast; mammary gland; adenocarcinoma; pleural effusion

Morphology: epithelial
Split Ratio: 1:3 to 1:6
Mycoplasma Test: negative
Cytogenetics: hypertriploid to hypotetraploid, modal number=82
Doubling time: 29 hrs
Receptor expressed: estrogen receptor, positive

Incubation Temp(°C): 37°C
Incubation Condition: 5% CO2
Culture Medium: Minimum essential medium Eagle with 2 mM L-glutamine and Earle's BSS adjusted to contain 1.5 g/L sodium bicarbonate, 0.1 mM non-essential amino acids and 1.0 mM sodium pyruvate,90%;fetal bovine serum,10%
Growth Property: adherent
SubCulture Procedure: Trypsin-EDTA
Freezed Medium: 93% culture medium + 7% DMSO
Medium Renewal: every 2 to 3 days

Description: the line retains the ability to process estradiol via cytoplasmic estrogen receptors and the capability of forming domes; it contains the Tx-4 oncogene; growth of MCF7 cells is inhibited by tumor necrosis factor alpha (TNF alpha); secretion of IGFBP's can be modulated by antiestrogens.
Others: STR-PCR profile — D7S820:8, 9 CSF1PO:10 TH01:6 D13S317:11 D16S539:11, 12 vWA:14, 15 TPOX:9, 12 Amelogenin:X D5S818:11, 12
Product Character: insulin-like growth factor binding proteins (IGFBP) BP-2; BP-4; BP-5

Image / Description:   100倍視野下低細胞密度

Biosafety Level: 1
Reference: J. Natl. Cancer Inst. 51:1409-1416,1973; Science 230: 943-945,1985
Frozen Vial for all NTD$ 6000 Order this item

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