Cell Line

BCRC Number: 60435
Name: U-937
Type: Human histiocytic lymphoma
Species: Homo sapiens (human)
Strain Ethnicity: Caucasian
Age Stage: 37 years
Gender: Male
Tissue: lymphoma; histiocytic; macrophage; histiocyte

Morphology: monocyte
Split Ratio: maintain cell density between 105 to 2x106 cells/ml
Isoenzyme Analysis: NP,G6PD,MD,LD
Mycoplasma Test: Negative
Cytogenetics: near triploid,modal number=58
Receptor expressed: complement (C3)

Incubation Temp(°C): 37°C
Incubation Condition: 5% CO2
Culture Medium: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate,90;fetal bovine serum,10%.
Growth Property: suspension
SubCulture Procedure: addition or replacement of medium
Freezed Medium: 93% culture medium + 7% DMSO
Seeding Density: 1-2 x 105 cells ml
Medium Renewal: Add fresh medium every 3 to 4 days (depending on cell density)

Description: derived from malignant cells obtained from the pleural effusion of a patient with histiocytic lymphoma; the cells express many of the monocyte-like characteristics exhibited by cells of histocytic origin; the cells bear receptors for Fc and C3, phagocytose antibody-coated erythrocytes and latex beads, stain strongly with nonspecific enzyme lysozyme; the cells lacks EB virus-related antigens and both surface and intracellular immunoglobulins; studies since 1979 have shown that U-937 cells can be induced to terminal monocytic differentiation by supernatants from human mixed lymphocyte cultures, phorbol esters, vitamin D3, gamma interferon, tumor necrosis factor (TNF) and, retinoic acid.
Others: STR-PCR profile — D7S820:9, 11 CSF1PO:12 TH01:6, 9.3 D13S317:10, 12 D16S539:12 vWA:14, 15 TPOX:8, 11 Amelogenin:X D5S818:12
Product Character: lysozyme; beta-2-microglobulin (beta 2 microglobulin); tumor necrosis factor (TNF), also known as tumor necrosis factor alpha (TNF-alpha, TNF alpha), after stimulation with phorbol myristic acid (PMA)

Image / Description:   100倍視野下高細胞密度

Biosafety Level: 1
Reference: Int. J. Cancer 17: 565-577, 1976; Nature 279: 328-331, 1979; In: Serrou, B.; Rosenfeld, C., eds. International Symposium on New Trends in Human Immunology and Cancer Chemotherapy. Paris: Dion; 1980:pp. 271-292; Nature 292: 848-850, 1981; Cancer Res. 43: 5
growing cell in 15ml tube for all NTD$ 6000 Order this item

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