Cell Line



BCRC Number: 60434
Name: Hep 3B2.1-7 (Hep 3B)
Type: Human hepatocellular carcinoma
Species: Homo sapiens (human)
Strain Ethnicity: Black
Age Stage: 8 years
Gender: Male
Tissue: liver; hepatocellular carcinoma

Morphology: epithelial
Split Ratio: 1:4 to 1:6
Isoenzyme Analysis: NP,G6PD,MD,LD
Mycoplasma Test: Negative
Cytogenetics: modal number=60 with a subtetraploid of 82
Tumorigenic: yes, forms tumors in nude mice

Incubation Temp(°C): 37°C
Culture Medium: Minimum essential medium Eagle with 2 mM L-glutamine and Earle's BSS adjusted to contain 1.5 g/L sodium bicarbonate, 0.1 mM non-essential amino acids, and 1.0 mM sodium pyruvate,90%;fetal bovine serum,10%.
Growth Property: adherent
SubCulture Procedure: trypsin-EDTA
Freezed Medium: 93% culture medium + 7% DMSO
Medium Renewal: every 2 to 3 days

Description: the line contains an integrated hepatitis B virus genome
Others: 此株細胞生長緩慢,冷凍管解凍後,培養於T25 flask,約需5-7天才長滿,細胞數目約有3-4 x 106 cells。 STR-PCR profile — D7S820:8 CSF1PO:8 TH01:6, 7 D13S317:12, 14 D16S539:10 vWA:17 TPOX:9 Amelogenin:X D5S818:13
Product Character: alpha fetoprotein (alpha-fetoprotein); hepatitis B surface antigen (HBsAg); albumin; alpha2 macroglobulin (alpha-2-macroglobulin); alpha1 antitrypsin (alpha-1-antitrypsin); transferrin;alpha1 antichymotrypsin (alpha-1-antichymotrypsin); haptoglobin; ceruloplasmin; plasminogen; complement (C3, C4); C3 activator; fibrinogen; alpha1 acid glycoprotein (alpha-1 acid glycoprotein);alpha2 HS glycoprotein (alpha-2-HS-glycoprotein); beta lipoprotein (beta-lipoprotein); retinol binding protein (retinol-binding protein); Gc globulin alpha1 antichymotrypsin (alpha-1-antichymotrypsin); haptoglobin

Image / Description:   200倍視野下低細胞密度
   200倍視野下高細胞密度
 

Biosafety Level: 2
Patent Statement: U.S. Pat. 4,393,133
Reference: Science 209: 497-499, 1980; U.S. Pat. 4,393,133 dated July 12, 1983; Nature 282: 615-616, 1979; In Vitro Cell. Dev. Biol. 23: 349-354, 1987
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