Cell Line

BCRC Number: 60432
Name: SW 872
Type: human liposarcoma
Species: Homo sapiens (human)
Strain Ethnicity: Caucasian
Age Stage: 36 years
Gender: Male
Tissue: liposarcoma

Morphology: epithelial
Split Ratio: 1:3 to 1:6
Isoenzyme Analysis: NP,G6PD,MD,LD
Mycoplasma Test: negative
Cytogenetics: hypertriploid; modal number = 80
Tumorigenic: yes, produces spindle cell sarcoma in nude mice consistent with liposarcoma

Incubation Temp(°C): 37°C
Culture Medium: Leibovitz's L-15 medium,90%; fetal bovine serum,10%.
Growth Property: adherent
SubCulture Procedure: trypsin-EDTA
Freezed Medium: 93% culture medium + 7% DMSO
Medium Renewal: every 2 to 3 days

Description: derived from a surgical specimen of a fibrosarcoma removed from a 36-year-old male Caucasian; the histopathology evaluation reported an undifferentiated malignant tumor consistent with liposarcoma
Others: STR-PCR profile — D7S820:8, 11 CSF1PO:10 TH01:8, 10 D13S317:11 D16S539:9, 12 vWA:17 TPOX:8, 11 Amelogenin:X D5S818:12, 13

Biosafety Level: 1
Reference: J. Natl. Cancer Inst. 58: 209-214, 1977
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